Detection of the satA gene and transferability of virginiamycin resistance in Enterococcus faecium from food-animals

FEMS Microbiol Lett. 1998 Nov 1;168(1):145-51. doi: 10.1111/j.1574-6968.1998.tb13267.x.

Abstract

The satA gene encoding streptogramin A resistance was detected in virginiamycin-resistant Enterococcus faecium isolates from pigs and broilers. The satA gene was present in 22 of 89 (25%) virginiamycin-resistant E. faecium isolates. It was shown that the satA gene and other gene(s) encoding streptogramin resistance could be transferred between isogenic E. faecium strains at frequencies ranging from 2.3 x 10(-4) to 2.2 x 10(-3) transconjugants per donor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / genetics*
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins*
  • Base Sequence
  • Chickens / microbiology
  • Conjugation, Genetic*
  • Drug Resistance, Microbial / genetics*
  • Electrophoresis
  • Electrophoresis, Gel, Pulsed-Field
  • Enterococcus faecium / drug effects*
  • Enterococcus faecium / genetics*
  • Enterococcus faecium / isolation & purification
  • Intestines / microbiology
  • Meat / microbiology
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / genetics
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA
  • Swine / microbiology
  • Virginiamycin / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Virginiamycin
  • Acetyltransferases
  • streptogramin A acetyltransferase, Enterococcus faecium