Abstract
To identify the proteins which may modulate the functions of peroxisome proliferator-activated receptor (PPAR), a rat liver cDNA library was screened by a yeast two-hybrid system, using the mouse PPARalpha as a bait. A protein named nuclear receptor binding factor-1 (NRBF-1) was identified, which interacts not only with PPARalpha, but also with various nuclear hormone receptors in the presence of the respective ligands. Both the hinge and ligand-binding domains of PPARalpha are required for the interaction. NRBF-1 seems to be translocated to the nucleus by a piggyback mechanism, together with PPARalpha. NRBF-1 has a significant homology to the yeast protein MRF1, a putative transcription factor regulating the expression of mitochondrial respiratory proteins. NRBF-1 might be another type of nuclear receptor co-operator.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites / genetics
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Biological Transport
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Carrier Proteins / physiology
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Cell Nucleus / chemistry
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Cell Nucleus / metabolism
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA-Binding Proteins*
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Intracellular Signaling Peptides and Proteins*
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Molecular Sequence Data
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Oxidoreductases
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Oxidoreductases Acting on CH-CH Group Donors
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Rats
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Carrier Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Nrbf1 protein, mouse
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Oxidoreductases
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Oxidoreductases Acting on CH-CH Group Donors
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trans-2-enoyl-CoA reductase (NADPH)