Abstract
Three open reading frames (ORFs) have been located downstream of cefE in the cephamycin C gene cluster of Streptomyces clavuligerus. ORF13 (pcd) encodes a 496-amino-acid protein (molecular weight [MW], 52,488) with an N-terminal amino acid sequence identical to that of pure piperideine-6-carboxylate dehydrogenase. ORF14 (cmcT) encodes a 523-amino-acid protein (MW, 54,232) analogous to Streptomyces proteins for efflux and resistance to antibiotics. ORF15 (pbp74) encodes a high molecular weight penicillin-binding protein (MW, 74, 094).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2-Aminoadipic Acid / biosynthesis*
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Amino Acid Sequence
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Bacterial Proteins*
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Cephamycins / metabolism*
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Cloning, Molecular
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DNA, Bacterial
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Molecular Sequence Data
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Multigene Family*
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Open Reading Frames
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Oxidoreductases Acting on CH-NH Group Donors / genetics*
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Proline / metabolism
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Sequence Homology, Amino Acid
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Streptomyces / genetics*
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Transcription, Genetic
Substances
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Bacterial Proteins
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Cephamycins
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DNA, Bacterial
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2-Aminoadipic Acid
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Proline
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Oxidoreductases Acting on CH-NH Group Donors
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delta-1-piperideine-6-carboxylate dehydrogenase
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cephamycin C