The pcd gene encoding piperideine-6-carboxylate dehydrogenase involved in biosynthesis of alpha-aminoadipic acid is located in the cephamycin cluster of Streptomyces clavuligerus

J Bacteriol. 1998 Sep;180(17):4753-6. doi: 10.1128/JB.180.17.4753-4756.1998.

Abstract

Three open reading frames (ORFs) have been located downstream of cefE in the cephamycin C gene cluster of Streptomyces clavuligerus. ORF13 (pcd) encodes a 496-amino-acid protein (molecular weight [MW], 52,488) with an N-terminal amino acid sequence identical to that of pure piperideine-6-carboxylate dehydrogenase. ORF14 (cmcT) encodes a 523-amino-acid protein (MW, 54,232) analogous to Streptomyces proteins for efflux and resistance to antibiotics. ORF15 (pbp74) encodes a high molecular weight penicillin-binding protein (MW, 74, 094).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Aminoadipic Acid / biosynthesis*
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Cephamycins / metabolism*
  • Cloning, Molecular
  • DNA, Bacterial
  • Molecular Sequence Data
  • Multigene Family*
  • Open Reading Frames
  • Oxidoreductases Acting on CH-NH Group Donors / genetics*
  • Proline / metabolism
  • Sequence Homology, Amino Acid
  • Streptomyces / genetics*
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Cephamycins
  • DNA, Bacterial
  • 2-Aminoadipic Acid
  • Proline
  • Oxidoreductases Acting on CH-NH Group Donors
  • delta-1-piperideine-6-carboxylate dehydrogenase
  • cephamycin C