During hibernation, variation in the metabolism of nerve cells occurs. Since the cytoskeleton plays an important role in nerve cell function, we have analyzed the immunocytochemical expression of two cytoskeleton components, i.e. phosphorylated 200 kDa neurofilament protein, and microtubule-associated protein 2 in the cerebellum and optic tectum of hibernating frogs (Rana esculenta) in comparison with active animals. In addition, we have considered the immunocytochemical expression of calmodulin, which is known to be involved in neurofilament phosphorylation. In hibernating animals, there was a decrease in the immunoreactivity for phosphorylated 200 kDa neurofilament protein and microtubule-associated protein 2 of fibers in both the cerebellum and in the optic tectum. In contrast, in the large neurons of the cerebellum, i.e. Purkinje neurons, there was an increase in the immunoreactivity for microtubule-associated protein 2. The changes in the cytoskeleton components were accompanied by a decrease in calmodulin immunoreactivity in the cytoplasm of nerve cells of the cerebellum. All the changes observed are consistent with a low neuronal activity during hibernation, as also indicated by previous microdensitometric and microfluorometric data. This shows a higher degree of chromatin condensation in hibernating animals and suggests that hibernation represents a simple form of neuronal plasticity.