Abstract
Immunoassays are widely used for determination of the concentration of steroid hormones. However, obtaining specific anti-steroid monoclonal antibodies remains difficult. We used antibody engineering and phage display methods to change the specificity of an anti-cortisol monoclonal antibody towards estradiol. This work demonstrates that production of recombinant antibodies may be a valuable way of obtaining the high-specificity antibodies required for steroid immunoassays.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenal Cortex Hormones / immunology*
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Amino Acid Substitution
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / genetics
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Antibodies, Monoclonal / immunology*
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Antibody Specificity
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Base Sequence
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Cross Reactions
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DNA Primers
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Estradiol / immunology*
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Hydrocortisone / immunology*
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Protein Engineering
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
Substances
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Adrenal Cortex Hormones
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Antibodies, Monoclonal
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DNA Primers
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Recombinant Proteins
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Estradiol
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Hydrocortisone