Omp85 proteins of Neisseria gonorrhoeae and Neisseria meningitidis are similar to Haemophilus influenzae D-15-Ag and Pasteurella multocida Oma87

Microb Pathog. 1998 Jul;25(1):11-21. doi: 10.1006/mpat.1998.0206.

Abstract

The genes encoding homologous 85 kDa outer membrane proteins of Neisseria gonorrhoeae and Neisseria meningitidis have been cloned and sequenced. The gonococcal gene, omp85, was identified by screening a genomic library with an antiserum raised against purified gonococcal outer membranes. The gene encoded a 792 amino acid protein, Omp85, having a typical signal peptide and a carboxyl-terminal phenylalanine characteristic of outer membrane proteins. The amino acid sequence was similar to that of the D15 protective surface antigen (D-15-Ag) of Haemophilus influenzae, and the Oma87 of Pasteurella multocida. Southern analysis demonstrated that omp85 was present as a single copy in N. gonorrhoeae and N. meningitidis. PCR amplification was used to obtain a clone of the N. meningitidis omp85 homologue. Sequence analysis revealed that the N. meningitidis Omp85 was 95% identical to the N. gonorrhoeae Omp85.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Outer Membrane Proteins / analysis
  • Bacterial Outer Membrane Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Genes, Bacterial / genetics
  • Haemophilus influenzae / chemistry*
  • Molecular Sequence Data
  • Neisseria gonorrhoeae / chemistry*
  • Neisseria meningitidis / chemistry*
  • Pasteurella multocida / chemistry*
  • Rabbits
  • Recombinant Fusion Proteins / biosynthesis
  • Sequence Analysis

Substances

  • Bacterial Outer Membrane Proteins
  • Oma87 protein, bacteria
  • Omp85 protein, Neisseria
  • Recombinant Fusion Proteins