Abstract
Expression of the Cdc6 protein (Cdc6p) is essential for formation of prereplicative complexes at budding yeast replication origins. Analysis of mutations in the conserved nucleoside triphosphate (NTP)-binding site of Cdc6p described here suggests that NTPs are required both for the productive interaction of Cdc6p with replication origins during G1 and the quantitative loading of the Mcm2-7 family of proteins onto chromatin. We show that Cdc6p exhibits significant sequence similarity to subunits of eukaryotic and prokaryotic clamp-loaders, which load ring-shaped DNA polymerase processivity factors onto DNA in an analogous reaction. Similarities in both sequence and mechanism suggest that Cdc6p and the clamp-loaders are members of a superfamily of nucleotide-dependent loading factors.
MeSH terms
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Alleles
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Amino Acid Sequence
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Binding Sites
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Cell Cycle Proteins / physiology*
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Cloning, Molecular
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DNA Footprinting
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DNA Replication*
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DNA, Fungal / biosynthesis
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DNA, Fungal / genetics*
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DNA-Binding Proteins / physiology
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Fungal Proteins / physiology*
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Genes, Dominant
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Homeodomain Proteins*
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Macromolecular Substances
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Minor Histocompatibility Antigens
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Molecular Sequence Data
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Multigene Family
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Nucleotides / metabolism
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Proto-Oncogene Proteins c-bcl-2*
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Recombinant Fusion Proteins / metabolism
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Replication Origin
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Replication Protein C
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Repressor Proteins*
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S Phase
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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BCL2-related protein A1
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CDC6 protein, S cerevisiae
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Cell Cycle Proteins
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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Homeodomain Proteins
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MATA1 protein, S cerevisiae
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Macromolecular Substances
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Minor Histocompatibility Antigens
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Nucleotides
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Proto-Oncogene Proteins c-bcl-2
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Recombinant Fusion Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Replication Protein C