Typing of Salmonella enteritidis of different phage types of PCR fingerprinting

N López-Molina; I Laconcha; A Rementería; A Audicana; I Perales; J Garaizar

doi:10.1046/j.1365-2672.1998.00425.x

Typing of Salmonella enteritidis of different phage types of PCR fingerprinting

J Appl Microbiol. 1998 May;84(5):877-82. doi: 10.1046/j.1365-2672.1998.00425.x.

Authors

N López-Molina¹, I Laconcha, A Rementería, A Audicana, I Perales, J Garaizar

Affiliation

¹ Microbiology Division, Health Department, Basque Government, Bilbao, Spain. oiblocan@vc.ehu.es

PMID: 9674142
DOI: 10.1046/j.1365-2672.1998.00425.x

Abstract

Fifty-nine isolates of Salmonella spp. were typed by PCR fingerprinting using three single primers: ERIC2, M13 and OPS-19. First, their discrimination power in a group of nine different serotypes were studied and considerable differences in the band patterns were obtained. Further, a panel of 51 isolates of Salmonella enteritidis with eight different phage types were analysed with the three primers. The discriminating power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types of Salm. enteritidis, but not all of them were differentiated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques*
Bacteriophage Typing
DNA Fingerprinting / methods*
DNA, Bacterial / analysis
Disease Outbreaks
Evaluation Studies as Topic
Humans
Polymerase Chain Reaction*
Salmonella Infections / epidemiology
Salmonella enteritidis / classification*
Salmonella enteritidis / genetics*
Serotyping

Substances

DNA, Bacterial