Adapting the Biomek 2000 Laboratory Automation Workstation for printing DNA microarrays

Biotechniques. 1998 Jul;25(1):106-10.

Abstract

The Biomek 2000 Laboratory Automation Workstation is used for liquid handling and other repetitive operations in many laboratories. Since it has very good spatial positioning capabilities, we have modified this workstation to deliver samples at high densities onto microscope slides to produce DNA microarrays. The workstation tool, originally designed for bacterial colony replication, was adapted to carry special printing pins and was further modified to improve its positional accuracy. Software written in the Tool Command Language was concurrently developed to control the movements of the workstation arm during the process of printing. With these modifications, the workstation can reliably deliver individual samples at a spacing of 0.5 mm, corresponding to a total of more than 3000 samples on a single slide. Arrays prepared in this way were successfully tested in hybridization experiments.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Technical Report

MeSH terms

  • Automation
  • DNA / analysis*
  • DNA / chemistry
  • DNA Probes / chemistry
  • DNA Probes / genetics
  • Data Display*
  • Equipment Design / instrumentation
  • Fluorescent Dyes
  • Image Processing, Computer-Assisted
  • Laboratories
  • Microchemistry
  • Microcomputers*
  • Nucleic Acid Hybridization
  • Programming Languages
  • Reproducibility of Results
  • Software

Substances

  • DNA Probes
  • Fluorescent Dyes
  • DNA