Abstract
Skeletal muscle tissue contains polymerase chain reaction (PCR) inhibitors that are coextracted by conventional nucleic acid extraction procedures. Myoglobin, a heme-containing molecule, was shown to act as a potent Thermus aquaticus DNA polymerase inhibitor and is likely to be involved in muscle tissue-associated PCR inhibition. The use of Thermus thermophilus DNA polymerase avoids muscle tissue-associated PCR inhibition, and should be used in case of small amounts or instability of the targeted nucleic acid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Viral / analysis
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DNA, Viral / isolation & purification
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DNA-Directed DNA Polymerase / metabolism
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DNA-Directed DNA Polymerase / pharmacology*
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Exodeoxyribonucleases / pharmacology
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Fibroblasts / chemistry
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Fibroblasts / enzymology
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Glucosephosphate Dehydrogenase / genetics
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Herpesvirus 1, Human / genetics
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Humans
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Muscle, Skeletal / chemistry*
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Muscle, Skeletal / enzymology
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Myocardium / chemistry
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Myocardium / enzymology
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Myoglobin / metabolism*
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Polymerase Chain Reaction / methods*
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RNA, Messenger / analysis
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RNA, Messenger / isolation & purification
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Taq Polymerase / antagonists & inhibitors*
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Taq Polymerase / pharmacology
Substances
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DNA, Viral
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Myoglobin
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RNA, Messenger
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Glucosephosphate Dehydrogenase
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DNA polymerase, Thermus thermophilus
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Taq Polymerase
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DNA-Directed DNA Polymerase
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Exodeoxyribonucleases