Role of ultraviolet A-induced oxidative DNA damage in apoptosis via loss of mitochondrial membrane potential and caspase-3 activation

Biochem Biophys Res Commun. 1998 Jun 29;247(3):693-6. doi: 10.1006/bbrc.1998.8869.

Abstract

UVA has been implicated in multistage photocarcinogenesis. HPLC analysis revealed that the 7,8-dihydro-8-oxo-2'-deoxyguanosine content of DNA in HL-60 cells increased immediately after UVA irradiation. Pulsed field gel electrophoresis showed that cellular DNA strand breakage was induced at 1 h after UVA irradiation. Flow cytometry showed that mitochondrial membrane potential (delta psi m) decreased time-dependently in cells exposed to UVA. The activity of caspase-3 and DNA ladder formation increased at 2 h after UVA irradiation. These results suggest that UVA irradiation induces oxidative DNA damage, followed by the loss of delta psi m and subsequent activation of caspase-3, resulting in apoptosis.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Apoptosis / radiation effects*
  • Caspase 3
  • Caspases*
  • Cysteine Endopeptidases / metabolism*
  • DNA Damage / genetics*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Electrophoresis, Gel, Pulsed-Field
  • Enzyme Activation / radiation effects
  • Flow Cytometry
  • HL-60 Cells
  • Humans
  • Membrane Potentials / radiation effects*
  • Mitochondria / radiation effects*
  • Oligopeptides / metabolism
  • Oxidative Stress / radiation effects
  • Ultraviolet Rays / adverse effects*

Substances

  • Oligopeptides
  • 8-Hydroxy-2'-Deoxyguanosine
  • CASP3 protein, human
  • Caspase 3
  • Caspases
  • Cysteine Endopeptidases
  • Deoxyguanosine