Mediterranean spotted fever, caused by Rickettsia conorii, is a tick-borne infection. Serum samples for screening R. conorii antibodies of professionally exposed persons in the Antalya region, on the Mediterranean coast of Turkey, were analyzed. R. conorii IgG antibodies were detected in 13 of 98 (13.3%) serum samples. Mediterranean spotted fever antibodies were found to be positive in 9.4% of the men and 15.2% of the women (a total of 13 cases). Only three cases had a history of fever and rash.