N-hydroxysuccinimide ester labeling 5'-aminoalkyl DNA oligomers: reaction conditions and purification

J Chromatogr A. 1998 May 8;806(1):93-5. doi: 10.1016/s0021-9673(98)00093-4.

Abstract

Difficulties were encountered in labeling 5'-aminoalkyl DNA oligomers with glycolketo electrophore N-hydroxysuccinimide esters in aqueous sodium bicarbonate (a common base for this purpose), followed by C18-silica reversed-phase high-performance liquid chromatography (HPLC) to achieve purification. The electrophore-labeled oligomers were not separated readily either from the hydrolyzed electrophore or from the starting oligomer. This problem was overcome by conducting the reaction with triethylamine as a base, organic washing the reaction mixtures after evaporation, and separating on a C18-poly(styrene-divinylbenzene) HPLC packing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetonitriles
  • Base Sequence
  • Chromatography, High Pressure Liquid* / methods
  • DNA / chemistry*
  • DNA / isolation & purification*
  • DNA Probes
  • Esters*
  • Hydrolysis
  • Molecular Structure
  • Sodium Bicarbonate
  • Succinimides*

Substances

  • Acetonitriles
  • DNA Probes
  • Esters
  • Succinimides
  • Sodium Bicarbonate
  • DNA
  • N-hydroxysuccinimide
  • acetonitrile