Abstract
A group of novel human Ran-binding proteins, RanBP3, was identified using the yeast two-hybrid system via Ran-mediated interaction with the nucleotide exchange factor RCC1. Several open reading frames, representing putative alternatively spliced products, were established by cDNA cloning. Two of them, RanBP3-a and RanBP3-b, encode nuclear hydrophilic proteins of 499 and 562 amino acid residues. The sequences contain FXFG motifs, characteristic of a subgroup of nucleoporins, and a C-terminal domain showing similarity to the Ran-binding protein RanBP1. These proteins are localized in the nucleus, preferentially bind RanGTP and may be nuclear effectors of the Ran pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Carrier Proteins / biosynthesis
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cell Cycle Proteins*
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Cell Nucleus / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism
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GTP-Binding Proteins / biosynthesis
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / genetics*
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GTP-Binding Proteins / metabolism
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Guanine Nucleotide Exchange Factors*
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HeLa Cells
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Humans
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Molecular Sequence Data
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Nucleocytoplasmic Transport Proteins*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Yeasts / metabolism
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ran GTP-Binding Protein*
Substances
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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Guanine Nucleotide Exchange Factors
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Nuclear Proteins
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Nucleocytoplasmic Transport Proteins
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RANBP3 protein, human
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RCC1 protein, human
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Recombinant Proteins
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ran-binding protein 1
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GTP-Binding Proteins
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ran GTP-Binding Protein
Associated data
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GENBANK/Y08697
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GENBANK/Y08698
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GENBANK/Y08699