Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining

S Ito; N Muguruma; S Hayashi; S Taoka; T Bando; K Inayama; M Sogabe; T Okahisa; S Okamura; H Shibata; T Irimura; K Takesako; S Shibamura

doi:10.1016/s0968-0896(98)00023-6

Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining

Bioorg Med Chem. 1998 May;6(5):613-8. doi: 10.1016/s0968-0896(98)00023-6.

Authors

S Ito¹, N Muguruma, S Hayashi, S Taoka, T Bando, K Inayama, M Sogabe, T Okahisa, S Okamura, H Shibata, T Irimura, K Takesako, S Shibamura

Affiliation

¹ Second Department of Internal Medicine, School of Medicine, University of Tokushima, Japan. ito@clin.med.tokushima-u.ac.jp

PMID: 9629475
DOI: 10.1016/s0968-0896(98)00023-6

Abstract

Fluorescence intensity of indocyanine green (ICG) derivative (ICG-sulfo-OSu) was too low for its use to detect microlesions. Therefore, we examined the effects of reinforcement agents on ICG-sulfo-OSu labeled antibodies. Solutions of distearoylphosphatic acid sodium salt (DSPA) and octylglucoside (OG) in physiological phosphate buffered saline (PBS) were found to increase the intensity of fluorescence of ICG-sulfo-OSu labeled antibodies, with shift in the fluorescence peak wavelength from 804 to 821 nm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / chemistry
Fluorescent Antibody Technique
Fluorescent Dyes / standards*
Indocyanine Green / analogs & derivatives*
Indocyanine Green / chemistry
Spectrophotometry, Infrared / methods*

Substances

Antibodies
Fluorescent Dyes
indocyanine green-sulfo-OSu
Indocyanine Green