Discrimination between different types of human adeno-associated viruses in clinical samples by PCR

J Virol Methods. 1998 Mar;71(1):17-25. doi: 10.1016/s0166-0934(97)00198-5.

Abstract

Persistent infection of human tissues with the helper virus-dependent parvovirus, adeno-associated virus (AAV) was detected by polymerase chain reaction (PCR) using primer pairs detecting AAV types 2, 3 or 5. In order to develop PCR protocols which discriminate between the different serotypes of AAV, the DNA of AAV-5 was sequenced partially and compared with the published sequences of AAV-2 and -3. Type specific oligonucleotides and specific probes which allow the distinction between human AAV types by PCR are described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers / genetics
  • DNA, Viral / genetics*
  • Dependovirus / classification*
  • Dependovirus / genetics*
  • Dependovirus / isolation & purification
  • Electrophoresis, Agar Gel
  • Electrophoresis, Polyacrylamide Gel
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sequence Alignment
  • Sequence Analysis
  • Sequence Analysis, DNA / methods*
  • Serotyping

Substances

  • DNA Primers
  • DNA, Viral