A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide

P M Desmarchelier; S S Bilge; N Fegan; L Mills; J C Vary Jr; P I Tarr

doi:10.1128/JCM.36.6.1801-1804.1998

A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide

J Clin Microbiol. 1998 Jun;36(6):1801-4. doi: 10.1128/JCM.36.6.1801-1804.1998.

Authors

P M Desmarchelier¹, S S Bilge, N Fegan, L Mills, J C Vary Jr, P I Tarr

Affiliation

¹ Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia. Patricia.Desmarchelier@foodscience.afisc.csiro.au

Abstract

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Typing Techniques
Carbohydrate Epimerases / genetics*
Enzyme-Linked Immunosorbent Assay
Escherichia coli O157 / genetics
Escherichia coli O157 / isolation & purification*
Humans
Immunoassay
Milk / microbiology*
O Antigens / biosynthesis*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Serotyping
Transaminases / genetics*

Substances

O Antigens
Transaminases
Carbohydrate Epimerases
perosamine synthetase