[Incorporation of neoglycolipids into K562 cells. Model for the study of carbohydrate dependent lysis of target cells by natural killer cells]

E I Kovalenko; M A Sablina; S V Khaĭdukov; E V Khirova; N V Bovin

[Incorporation of neoglycolipids into K562 cells. Model for the study of carbohydrate dependent lysis of target cells by natural killer cells]

Bioorg Khim. 1998 Mar;24(3):224-8.

[Article in Russian]

Authors

E I Kovalenko¹, M A Sablina, S V Khaĭdukov, E V Khirova, N V Bovin

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

PMID: 9612565

Abstract

A model system was developed to study the role of cell surface oligosaccharides in cytotoxicity mediated by natural killer (NK) cells. Polymeric glycoconjugates Glyc-PAA-PE and Glyc-PAA(Flu)-PE (where Glyc is the 3-aminopropyl Le(x) residue, PAA is the poly(N-2-hydroxyethylacrylamide) matrix, Flu is the residue of a fluorescein derivative, and PE is the phosphatidylethanolamine residue) were synthesized, and their association with the membranes of living K562 cells was studied.

MeSH terms

Cell Membrane / metabolism
Cytotoxicity Tests, Immunologic
Flow Cytometry
Fluorescein / chemistry
Glycolipids / chemistry
Glycolipids / metabolism*
Humans
Killer Cells, Natural / metabolism*
Lewis X Antigen / metabolism*
Oligosaccharides / chemistry
Oligosaccharides / metabolism*
Phosphatidylethanolamines / chemistry
Tumor Cells, Cultured

Substances

Glycolipids
Lewis X Antigen
Oligosaccharides
Phosphatidylethanolamines
Fluorescein