Abstract
We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elaphus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA Fingerprinting / veterinary*
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DNA Primers / chemistry
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DNA-Binding Proteins / genetics*
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Deer / genetics*
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Electrophoresis, Agar Gel / veterinary
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Female
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Fragile X Mental Retardation Protein
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Fragile X Syndrome / genetics
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Genetic Markers
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Male
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nuclear Proteins*
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Polymerase Chain Reaction / veterinary*
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RNA-Binding Proteins*
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Sequence Analysis, DNA / veterinary
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Sex Determination Analysis / veterinary*
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Sex-Determining Region Y Protein
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Transcription Factors*
Substances
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DNA Primers
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DNA-Binding Proteins
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Genetic Markers
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Nerve Tissue Proteins
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Nuclear Proteins
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RNA-Binding Proteins
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Sex-Determining Region Y Protein
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Transcription Factors
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Fragile X Mental Retardation Protein