Sex identification of elk (Cervus elaphus canadensis), moose (Alces alces), and white-tailed deer (Odocoileus virginianus) using the polymerase chain reaction

J Forensic Sci. 1998 May;43(3):477-82.

Abstract

We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elaphus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Fingerprinting / veterinary*
  • DNA Primers / chemistry
  • DNA-Binding Proteins / genetics*
  • Deer / genetics*
  • Electrophoresis, Agar Gel / veterinary
  • Female
  • Fragile X Mental Retardation Protein
  • Fragile X Syndrome / genetics
  • Genetic Markers
  • Male
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nuclear Proteins*
  • Polymerase Chain Reaction / veterinary*
  • RNA-Binding Proteins*
  • Sequence Analysis, DNA / veterinary
  • Sex Determination Analysis / veterinary*
  • Sex-Determining Region Y Protein
  • Transcription Factors*

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Genetic Markers
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • RNA-Binding Proteins
  • Sex-Determining Region Y Protein
  • Transcription Factors
  • Fragile X Mental Retardation Protein