Multiple staining was performed on resected tumors. Samples after curative surgery in 40 cases of bile duct carcinoma for observation of tumor blood vessel density (TVD), and perineural invasion index (PNI). Lymphatic invasion was found in 29 cases (72.5%), blood vessel invasion in 31 (77.5%), and PNI in 33 (82.5%). TVD and PNI were positively correlated with metastasis. Three years after operation, the recurrent rate was 100%, with an averaging recurrent time of 9.6 months in the metastatic group in contrast to 64.3% and 17.5 months in those without metastasis.