Abstract
Five different isoforms of cytochrome P450 including 1A1, 1A2, 2E1, 2A and 2B6 have been identified in human glioma Hs 683 cell line using RT-PCR reaction. These isoforms belong to four distinct subfamilies. The effect of benzanthracene (Ba) as inducer was tested on the mRNA level of cytochrome P450 1A1. Northern blot analysis clearly showed an induction response from these cells to Ba in a proportion that is comparable to the induction seen in rat glioma cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aryl Hydrocarbon Hydroxylases*
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Base Sequence
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Benz(a)Anthracenes / pharmacology
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Blotting, Northern
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Blotting, Western
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Cytochrome P-450 CYP1A1 / chemistry
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Cytochrome P-450 CYP1A1 / genetics
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Cytochrome P-450 CYP1A2 / chemistry
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Cytochrome P-450 CYP1A2 / genetics
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Cytochrome P-450 CYP2B6
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Cytochrome P-450 CYP2E1 / chemistry
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Cytochrome P-450 CYP2E1 / genetics
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Cytochrome P-450 Enzyme System / chemistry
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Cytochrome P-450 Enzyme System / genetics*
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Gene Expression / genetics
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Glioma / chemistry
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Glioma / enzymology*
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Glioma / ultrastructure
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Humans
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Isoenzymes / chemistry
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Isoenzymes / genetics*
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Microsomes / drug effects
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Microsomes / enzymology
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Oxidoreductases, N-Demethylating / chemistry
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Oxidoreductases, N-Demethylating / genetics
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Polymerase Chain Reaction
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Steroid Hydroxylases / chemistry
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Steroid Hydroxylases / genetics
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Tumor Cells, Cultured
Substances
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Benz(a)Anthracenes
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DNA, Complementary
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Isoenzymes
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RNA, Messenger
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Cytochrome P-450 Enzyme System
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benz(a)anthracene
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Steroid Hydroxylases
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Cytochrome P-450 CYP2E1
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Aryl Hydrocarbon Hydroxylases
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CYP2B6 protein, human
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Cytochrome P-450 CYP1A1
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Cytochrome P-450 CYP1A2
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Cytochrome P-450 CYP2B6
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steroid hormone 6-beta-hydroxylase
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Oxidoreductases, N-Demethylating