Transcription of multiple cell wall protein-encoding genes in Saccharomyces cerevisiae is differentially regulated during the cell cycle

FEMS Microbiol Lett. 1998 Apr 15;161(2):345-9. doi: 10.1111/j.1574-6968.1998.tb12967.x.

Abstract

The yeast cell wall consists of an internal skeletal layer and an outside protein layer. The synthesis of both beta-1,3-glucan and chitin, which together from the cell wall skeleton, is cell cycle-regulated. We show here that the expression of five cell wall protein-encoding genes (CWP1, CWP2, SED1, TIP1 and TIR1) is also cell cycle-regulated. TIP1 is expressed in G1 phase, CWP1, CWP2 and TIR1 are expressed in S/G2 phase, and SED1 in M phase. The data suggest that these proteins fulfil distinct functions in the cell wall.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cell Cycle / genetics
  • Cell Wall / chemistry
  • Cell Wall / genetics
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal / genetics*
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Phenotype
  • RNA, Messenger / isolation & purification
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic / genetics*

Substances

  • Fungal Proteins
  • Membrane Proteins
  • RNA, Messenger