We have previously described the increase of microsomal lipid peroxidation and the appearance of new oxidized forms of Cu,Zn-superoxide dismutase in the liver of gilthead seabrams (Sparus aurata) injected with model xenobiotics, due to the increased production of reactive oxygen species (ROS) (Pedrajas et al., Chem. Biol. Interact., 1995). The effects of dieldrin and copper(II) on subcellular organelles directly related with ROS production are now studied. Immature fish were injected with dieldrin and copper, 0.15 and 1.0 mg/ kg, respectively. After 2 and 7 days, the livers were homogenized and the catalase and superoxide dismutase activities were determined in subcellular fractions isolated by differential centrifugation: nucleus, mitochondria, light mitochondrial fraction (LMF), microsomes and cytosolic fraction. Peroxisomes were isolated from LMF by discontinuous gradient centrifugation using Nycodenz. Changes in catalase and superoxide dismutase depended on the xenobiotic and affected to different subcellular fractions. Thus, the effects of copper(II) were mainly in nucleus and cytosol, whereas dieldrin induced catalase and superoxide dismutase (up to 2.8-fold) preferentially in nucleus and LMF fractions. Dieldrin-injected fish showed also highly increased activity of palmitoyl-CoA-oxidase (9.3-fold) and a nearly 2-fold increase in the protein concentration of the peroxisomal fraction. The results described above suggest that the oxidative stress previously detected for dieldrin in fish liver affects also to the peroxisomal enzymes.