Abstract
We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Bacillus subtilis / chemistry
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Bacterial Proteins / analysis*
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Erythrocyte Membrane / chemistry*
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Erythrocyte Membrane / parasitology
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Erythrocyte Membrane / ultrastructure
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Green Fluorescent Proteins
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Indicators and Reagents
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Luminescent Proteins
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Membrane Proteins / analysis*
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Microscopy, Confocal
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Microscopy, Electron, Scanning
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Microscopy, Fluorescence / instrumentation*
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Microscopy, Fluorescence / methods*
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Optics and Photonics
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Plasmodium falciparum
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Protozoan Proteins / analysis*
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Sensitivity and Specificity
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Spores, Bacterial / chemistry
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Spores, Bacterial / ultrastructure
Substances
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Bacterial Proteins
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Indicators and Reagents
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Luminescent Proteins
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Membrane Proteins
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Protozoan Proteins
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Green Fluorescent Proteins