A novel long-acting VIP analog in the guinea pig trachea in vitro

Peptides. 1998;19(3):593-7. doi: 10.1016/s0196-9781(97)00457-9.

Abstract

The relaxant effect of a novel VIP analog, [Arg15,20,21Leu17]-VIP-Gly-Lys-Arg-NH2 was compared with that of the original VIP in the same guinea pig trachea precontracted by carbachol in vitro. The VIP analog caused significantly and concentration-dependent relaxation similarly to the original VIP. In contrast to the original VIP, the VIP analog demonstrated a slow onset and offset of action, with more than 90% of its maximum relaxation remaining 6 h after administration. Peptidase inhibition by captopril and phosphoramidon increased the relaxant effect and duration of action for original VIP but not for the VIP analog.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albuterol / pharmacology
  • Amino Acid Sequence
  • Animals
  • Captopril / pharmacology
  • Carbachol / pharmacology
  • Guinea Pigs
  • Male
  • Molecular Sequence Data
  • Muscle Relaxation / drug effects
  • Structure-Activity Relationship
  • Time Factors
  • Trachea / drug effects*
  • Vasoactive Intestinal Peptide / analogs & derivatives*
  • Vasoactive Intestinal Peptide / chemistry
  • Vasoactive Intestinal Peptide / metabolism
  • Vasoactive Intestinal Peptide / pharmacology*

Substances

  • Arg(15,20,21)-Leu(17)-VIP-Gly-Lys-Arg-NH2
  • Vasoactive Intestinal Peptide
  • Carbachol
  • Captopril
  • Albuterol