Nucleotide sequence and molecular characterization of a gene encoding GTP-binding protein from Streptococcus gordonii

FEMS Microbiol Lett. 1997 Nov 15;156(2):211-6. doi: 10.1111/j.1574-6968.1997.tb12729.x.

Abstract

A 1286-bp fragment of chromosomal DNA from Streptococcus gordonii strain Challis was cloned and sequenced. The gene sgg consisted of 897-bp nucleotides encoding a 299-amino acid polypeptide (33,200 Da). The deduced amino acid sequence exhibited significant similarity to Era, G protein of Escherichia coli. The nucleotide binding assay demonstrated that recombinant Sgg bound [32P]GTP but not [32P]ATP, [32P]CTP, or [32P]UTP. These findings indicate that Sgg is a member of the G protein superfamily in the genus Streptococcus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / analysis
  • GTP-Binding Proteins / genetics*
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Molecular Sequence Data
  • Recombinant Proteins / analysis
  • Sequence Analysis, DNA
  • Streptococcus / genetics*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Recombinant Proteins
  • GTP-Binding Proteins
  • SGG protein, Streptococcus

Associated data

  • GENBANK/AB003804