DNA loop anchorage region colocalizes with the replication origin located downstream to the human gene encoding lamin B2

J Cell Biochem. 1998 Apr 1;69(1):13-8. doi: 10.1002/(sici)1097-4644(19980401)69:1<13::aid-jcb2>3.0.co;2-y.

Abstract

The recently developed procedure of topoisomerase II-mediated DNA loop excision has been used to analyze the topological organization of a human genome fragment containing the gene encoding lamin B2 and the ppv1 gene. A 3.5 kb long DNA loop anchorage/topoisomerase II cleavage region was found within the area under study. This region includes the end of the lamin B2 coding unit and an intergenic region where an origin of DNA replication was previously found. These observations further corroborate the hypothesis that DNA replication origins are located at or close to DNA loop anchorage regions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / genetics
  • Chromosome Mapping
  • DNA / chemistry*
  • DNA / genetics*
  • DNA / metabolism
  • DNA Replication / genetics
  • DNA Topoisomerases, Type II / metabolism
  • Genome, Human
  • HL-60 Cells
  • Humans
  • Lamin Type B*
  • Lamins
  • Nuclear Proteins / genetics*
  • Nucleic Acid Conformation
  • Replication Origin / genetics*

Substances

  • Lamin Type B
  • Lamins
  • Nuclear Proteins
  • DNA
  • DNA Topoisomerases, Type II