Abstract
Purification and properties of transcription factor IIIA (TF IIIA) from maize pollen (Zea mays L.) are presented for the first time. The purified protein has a molecular mass of about 35 kDa and exhibits binding affinity toward both 5S rRNA and 5S rRNA gene. It also facilitates transcription of the 5S rRNA gene in a HeLa cell extract.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cotyledon / metabolism*
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DNA-Binding Proteins / isolation & purification*
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DNA-Binding Proteins / metabolism
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HeLa Cells
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Humans
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Pollen / metabolism
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RNA, Ribosomal, 5S / genetics
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Transcription Factor TFIIIA
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Transcription Factors / isolation & purification*
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Transcription Factors / metabolism
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Transcription, Genetic
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Zea mays / metabolism*
Substances
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DNA-Binding Proteins
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RNA, Ribosomal, 5S
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Transcription Factor TFIIIA
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Transcription Factors