Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein

J Virol Methods. 1997 Dec;69(1-2):181-9. doi: 10.1016/s0166-0934(97)00158-4.

Abstract

Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Viral / biosynthesis*
  • Antibodies, Viral / immunology
  • Blotting, Western
  • Capsid / chemistry
  • Capsid / immunology*
  • Capsid Proteins*
  • Cloning, Molecular
  • Enzyme-Linked Immunosorbent Assay
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology*
  • Plant Diseases / virology
  • Plant Extracts
  • Potyvirus / immunology*
  • Potyvirus / isolation & purification*
  • Sequence Analysis
  • Species Specificity

Substances

  • Antibodies, Viral
  • Capsid Proteins
  • Peptide Fragments
  • Plant Extracts
  • coat protein, Plum pox virus

Associated data

  • GENBANK/Y09851