High-performance liquid chromatographic determination of ritonavir in human plasma, cerebrospinal fluid and saliva

J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):119-26. doi: 10.1016/s0378-4347(97)00500-8.

Abstract

A simple, ion-pair high-performance liquid chromatographic method has been developed and validated for the quantitative determination of the HIV protease inhibitor ritonavir in human plasma, cerebrospinal fluid and saliva. Sample pretreatment consisted of precipitation of proteins with acetonitrile prior to high-performance liquid chromatography with ultraviolet detection at 239 nm. The method has been validated over the range of 50 ng/ml to 50 microg/ml with use of 100-microl volumes of sample. The currently described assay has been used successfully for the analysis of ritonavir in plasma, cerebrospinal fluid and saliva in HIV-1 infected patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / blood
  • Acquired Immunodeficiency Syndrome / cerebrospinal fluid
  • Acquired Immunodeficiency Syndrome / metabolism
  • Chromatography, High Pressure Liquid
  • HIV Protease Inhibitors / analysis*
  • HIV Protease Inhibitors / blood
  • HIV Protease Inhibitors / cerebrospinal fluid
  • HIV Protease Inhibitors / pharmacokinetics
  • HIV-1
  • Humans
  • Reproducibility of Results
  • Ritonavir / analysis*
  • Ritonavir / blood
  • Ritonavir / cerebrospinal fluid
  • Ritonavir / pharmacokinetics
  • Saliva / chemistry*
  • Sensitivity and Specificity

Substances

  • HIV Protease Inhibitors
  • Ritonavir