Results of cultivation of newborn rat keratinocytes are presented, differences between growing rat and human keratinocytes in culture being revealed in addition to possibilities of experimental use of these results. It has been shown that a rat keratinocyte layer forms faster than a human one: the former it is thinner and contains twice less layers. The main peculiarity of rat keratinocytes in culture is their ability to grow successfully without feeder cells, while the presence of feeder cells is crucial for human keratinocyte growth in culture.