Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by iron deficiency in barley roots

K Suzuki; R Itai; K Suzuki; H Nakanishi; N K Nishizawa; E Yoshimura; S Mori

doi:10.1104/pp.116.2.725

Formate dehydrogenase, an enzyme of anaerobic metabolism, is induced by iron deficiency in barley roots

Plant Physiol. 1998 Feb;116(2):725-32. doi: 10.1104/pp.116.2.725.

Authors

K Suzuki¹, R Itai, K Suzuki, H Nakanishi, N K Nishizawa, E Yoshimura, S Mori

Affiliation

¹ Department of Applied Biological Chemistry, University of Tokyo, Japan.

Abstract

To identify the proteins induced by Fe deficiency, we have compared the proteins of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-dimensional polyacrylamide gel electrophoresis. Peptide sequence analysis of induced proteins revealed that formate dehydrogenase (FDH), adenine phosphoribosyltransferase, and the lds3 gene product (for Fe deficiency-specific) increased in Fe-deficient roots. FDH enzyme activity was detected in Fe-deficient roots but not in Fe-sufficient roots. A cDNA encoding FDH (Fdh) was cloned and sequenced. Fdh expression was induced by Fe deficiency. Fdh was also expressed under anaerobic stress and its expression was more rapid than that induced by Fe deficiency. Thus, the expression of Fdh observed in Fe-deficient barley roots appeared to be a secondary effect caused by oxygen deficiency in Fe-deficient plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
DNA, Complementary
Electrophoresis, Gel, Two-Dimensional
Enzyme Induction
Formate Dehydrogenases / biosynthesis*
Formate Dehydrogenases / genetics
Hordeum / enzymology*
Iron / metabolism*
Molecular Sequence Data
Plant Roots / enzymology*
Sequence Homology, Amino Acid

Substances

DNA, Complementary
Iron
Formate Dehydrogenases

Associated data

GENBANK/D88272