The change in aortic blood density in an in vivo rabbit preparation was measured to assess fluid movement at the pulmonary capillaries caused by infusion of hypertonic solution (NaCl, urea, glucose, sucrose, or raffinose in isotonic saline) into the vena cava over 20 s. The hypertonic disturbance increased the plasma osmotic pressure by </=30 mosmol/l. The density change indicates that the fluid extraction from the lung tissue was completed within 10 s. It was followed by a fluid filtration into the lung tissue and then an extraction and filtration from peripheral organs. An exchange model with flow dispersion yields two equations to estimate the osmotic conductance (sigmaK; where sigma is the reflection coefficient of the test solute and K is the filtration coefficient including the total capillary surface area), and the tissue fluid volume from the area and first moment of the measured density change over the extraction phase. The values of sigmaK are 1.40 +/- 0.11, 1.00 +/- 0. 10, 1.71 +/- 0.10, 2.60 +/- 0.23, and 3.73 +/- 0.34 (SE) ml . h-1 . mosmol-1 . l . g-1 for NaCl, urea, glucose, sucrose, and raffinose, respectively. Consistent with the model prediction, the tissue fluid volume (0.28 +/- 0.04 ml/g wet lung tissue) was independent of the solute used. This value suggests that all fluid spaces in the alveolar septa participate in the process of fluid extraction due to an increase in plasma osmotic pressure.