Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination

J Biomol NMR. 1997 Dec;10(4):397-401. doi: 10.1023/a:1018332327565.

Abstract

We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Cadmium
  • DNA-Binding Proteins / chemistry*
  • Magnetic Resonance Spectroscopy
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / chemistry*

Substances

  • DNA-Binding Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Cadmium