Expression and imprinting of the insulin-like growth factor II gene in neonatal mouse cerebellum

J Neurosci Res. 1997 Dec 15;50(6):958-66. doi: 10.1002/(SICI)1097-4547(19971215)50:6<958::AID-JNR6>3.0.CO;2-C.

Abstract

Insulin-like growth factor II (IGF-II) plays significant roles in the growth and development of mammals through the regulation of mitogenesis and cell survival. Previously, IGF-II mRNA transcripts within the CNS were detected in the choroid plexus and leptomeninges (DeChiara et al., 1991). The objective of this study was to determine the expression pattern of IGF-II mRNA in different cell types of the cerebellum during development. We report here that the IGF-II gene is transcribed in granule and glial cells within the cerebellar parenchyma at various times during the early postnatal period in mice. IGF-II gene expression is further regulated by parent-specific imprinting such that only the paternal IGF-II allele is expressed in granule cells. In contrast, choroid plexus and leptomeninges express IGF-II mRNAs biallelically, indicating that cell type-specific regulation of genomic imprinting occurs within the mammalian CNS.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Cells, Cultured
  • Cerebellum / cytology
  • Cerebellum / metabolism*
  • Choroid Plexus / metabolism
  • Gene Expression Regulation / physiology*
  • Genomic Imprinting*
  • Insulin-Like Growth Factor II / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Neurons / metabolism
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis
  • Transcription, Genetic

Substances

  • RNA, Messenger
  • Insulin-Like Growth Factor II