A short GC-rich sequence involved in deletion formation of cloned DNA in E. coli

Acta Biol Hung. 1997;48(3):275-9.

Abstract

A spontaneous deletion probably caused by rec A independent recombination between short-direct repeat sequences was observed in pUC19 plasmid carrying a piece of Streptomyces genomic DNA after culturing in liquid medium. The deletion removed an unknown portion of the cloned DNA and the BamHI-EcoRI part of the multiple cloning site with an additional flanking 111 bp from the vector. At the junction a 13 bp GC-rich DNA sequence highly homologous to a known spontaneous deletion hotspot was found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Genetic Vectors
  • Molecular Sequence Data
  • Plasmids / genetics
  • Rec A Recombinases / genetics
  • Recombination, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Sequence Deletion
  • Streptomyces griseus / genetics

Substances

  • DNA, Bacterial
  • Rec A Recombinases