Abstract
About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.
MeSH terms
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Carbon Radioisotopes / chemistry
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Fructose / chemistry
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Fructose / metabolism
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Glucosyltransferases / genetics
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Glucosyltransferases / metabolism*
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Isotope Labeling / methods
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sucrose / chemical synthesis*
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Sucrose / metabolism
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Uridine Diphosphate Glucose / chemistry
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Uridine Diphosphate Glucose / metabolism
Substances
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Carbon Radioisotopes
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Recombinant Proteins
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Fructose
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Sucrose
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Glucosyltransferases
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sucrose synthase
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Uridine Diphosphate Glucose