Synthesis of asymmetrically labeled sucrose by a recombinant sucrose synthase

Biosci Biotechnol Biochem. 1997 Nov;61(11):1955-6. doi: 10.1271/bbb.61.1955.

Abstract

About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[14C]glucose or [14C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.

MeSH terms

  • Carbon Radioisotopes / chemistry
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Fructose / chemistry
  • Fructose / metabolism
  • Glucosyltransferases / genetics
  • Glucosyltransferases / metabolism*
  • Isotope Labeling / methods
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sucrose / chemical synthesis*
  • Sucrose / metabolism
  • Uridine Diphosphate Glucose / chemistry
  • Uridine Diphosphate Glucose / metabolism

Substances

  • Carbon Radioisotopes
  • Recombinant Proteins
  • Fructose
  • Sucrose
  • Glucosyltransferases
  • sucrose synthase
  • Uridine Diphosphate Glucose