Abstract
Residues 12-34 of mature human pulmonary surfactant protein B (SP-B1-78) are 68% homologous to residues 48-72 of the frog peptide antibiotic dermaseptin b I. We examined the effects of SP-B1-78 on the growth of Escherichia coli in order to find whether full length SP-B1-78 might act as a peptide antibiotic. We found that SP-B1-78 peptide inhibited growth of E. coli (MIC = 210 micrograms.ml-1), but that the SP-B variant [R/K-->S]SP-B1-78 was less potent (MIC = 500 micrograms.ml-1).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Analysis of Variance
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Escherichia coli / drug effects*
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Escherichia coli / growth & development
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Growth Inhibitors / pharmacology*
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Molecular Sequence Data
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Peptide Fragments / pharmacology*
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Proteolipids / chemistry
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Proteolipids / pharmacology*
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Pulmonary Surfactants / chemistry
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Pulmonary Surfactants / pharmacology*
Substances
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Growth Inhibitors
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Peptide Fragments
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Proteolipids
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Pulmonary Surfactants