Effects of non-glycated and glycated glucagon-like peptide-1(7-36) amide on glucose metabolism in isolated mouse abdominal muscle

Peptides. 1997;18(9):1327-33. doi: 10.1016/s0196-9781(97)00201-5.

Abstract

This study investigated the actions of non-glycated and glycated glucagon-like peptide-1(7-36)amide (tGLP-1) on glucose uptake and metabolism in isolated mouse abdominal muscle. Monoglycated tGLP-1 (Mr 3463.8) was prepared under hyperglycemic reducing conditions and purified by HPLC. Non-glycated tGLP-1 (10(-10)-10(-8) mol/l) stimulated both 2-deoxy-D-[1-3H]glucose uptake (1.3-1.5 fold) and 14C-glucose oxidation (1.4-1.7 fold) in muscle compared to controls without tGLP-1. Glycation reduced these stimulatory effects by 27-33% and 25% (at 10(-9) mol/l), respectively. tGLP-1 (10(-10)-10(-8) mol/l) promoted muscle glycogenesis and lactate production, whereas glycated peptide was ineffective below 10(-9) mol/l. This study demonstrates that tGLP-1 has potent glycogenic effects in mouse abdominal muscle in vitro and that glycation impairs its action.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdominal Muscles / drug effects*
  • Abdominal Muscles / metabolism
  • Animals
  • Glucagon
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptides
  • Glucose / metabolism*
  • Glycosylation
  • In Vitro Techniques
  • Insulin / pharmacology
  • Male
  • Mice
  • Oxidation-Reduction
  • Peptide Fragments
  • Peptides / pharmacology*

Substances

  • Insulin
  • Peptide Fragments
  • Peptides
  • Glucagon-Like Peptides
  • Glucagon-Like Peptide 1
  • Glucagon
  • Glucose
  • glucagon-like peptide 1 (7-37)