The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids

R Agusti; A S Couto; O E Campetella; A C Frasch; R M de Lederkremer

doi:10.1093/glycob/7.6.731

The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids

Glycobiology. 1997 Sep;7(6):731-5. doi: 10.1093/glycob/7.6.731.

Authors

R Agusti¹, A S Couto, O E Campetella, A C Frasch, R M de Lederkremer

Affiliation

¹ CIHIDECAR, Departmento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

PMID: 9376675
DOI: 10.1093/glycob/7.6.731

Abstract

The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, Thin Layer
Glycosylphosphatidylinositols / metabolism*
Membrane Lipids / metabolism*
Mice
Neuraminidase / metabolism*
Phosphatidylinositol Diacylglycerol-Lyase
Phosphoinositide Phospholipase C
Substrate Specificity
Trypanosoma cruzi / enzymology*
Type C Phospholipases / metabolism

Substances

Glycosylphosphatidylinositols
Membrane Lipids
Type C Phospholipases
Phosphoinositide Phospholipase C
Neuraminidase
Phosphatidylinositol Diacylglycerol-Lyase