Purification and characterization of transcription factor IIIA from higher plants

Eur J Biochem. 1997 Oct 1;249(1):107-12. doi: 10.1111/j.1432-1033.1997.t01-2-00107.x.

Abstract

Transcription factor IIIA (TF IIIA) binds and specifically activates transcription of eukaryotic 5S rRNA genes. It also forms a 7S ribonucleoprotein complex with mature 5S rRNA. Here, we describe the purification and properties of pTF IIIA from higher plants. The purified protein from tulip (Tulipa whittalii) has a molecular mass of about 40 kDa and also binds 5S rRNA and 5S rRNA genes. pTF IIIA also facilitates the transcription of a 5S rRNA gene in a HeLa cell extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Plant / genetics
  • DNA, Plant / metabolism
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal / metabolism
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism*
  • Genes, Plant
  • HeLa Cells
  • Humans
  • Molecular Weight
  • Plant Proteins / isolation & purification
  • Plant Proteins / metabolism
  • Plants / genetics
  • Plants / metabolism*
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • RNA, Ribosomal, 5S / genetics
  • RNA, Ribosomal, 5S / metabolism
  • Ribonucleoproteins / metabolism
  • Transcription Factor TFIIIA
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism*
  • Transcription, Genetic

Substances

  • DNA, Plant
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Plant Proteins
  • RNA, Plant
  • RNA, Ribosomal, 5S
  • Ribonucleoproteins
  • Transcription Factor TFIIIA
  • Transcription Factors