Abstract
Transcription factor IIIA (TF IIIA) binds and specifically activates transcription of eukaryotic 5S rRNA genes. It also forms a 7S ribonucleoprotein complex with mature 5S rRNA. Here, we describe the purification and properties of pTF IIIA from higher plants. The purified protein from tulip (Tulipa whittalii) has a molecular mass of about 40 kDa and also binds 5S rRNA and 5S rRNA genes. pTF IIIA also facilitates the transcription of a 5S rRNA gene in a HeLa cell extract.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Plant / genetics
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DNA, Plant / metabolism
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DNA, Ribosomal / genetics
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DNA, Ribosomal / metabolism
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DNA-Binding Proteins / isolation & purification*
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DNA-Binding Proteins / metabolism*
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Genes, Plant
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HeLa Cells
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Humans
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Molecular Weight
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Plant Proteins / isolation & purification
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Plant Proteins / metabolism
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Plants / genetics
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Plants / metabolism*
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RNA, Plant / genetics
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RNA, Plant / metabolism
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RNA, Ribosomal, 5S / genetics
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RNA, Ribosomal, 5S / metabolism
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Ribonucleoproteins / metabolism
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Transcription Factor TFIIIA
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Transcription Factors / isolation & purification*
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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DNA, Plant
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DNA, Ribosomal
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DNA-Binding Proteins
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Plant Proteins
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RNA, Plant
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RNA, Ribosomal, 5S
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Ribonucleoproteins
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Transcription Factor TFIIIA
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Transcription Factors