Abstract
Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / genetics
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Bacterial Proteins*
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Brassica / genetics*
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Cloning, Molecular / methods*
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Deoxyribonucleases, Type II Site-Specific
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Genes, Plant / genetics*
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Genetic Vectors / genetics*
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Glycoproteins / genetics*
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Plant Proteins / genetics*
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Plasmids / genetics
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Protein Kinases / genetics
Substances
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Bacterial Proteins
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Glycoproteins
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Plant Proteins
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self-incompatibility locus specific glycoprotein, Brassica
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Protein Kinases
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S-receptor kinase
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endodeoxyribonuclease MluI
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Deoxyribonucleases, Type II Site-Specific