Crystallization and preliminary crystallographic analysis of the sarcosine oxidase from Bacillus sp. NS-129

T Ichikawa; H Sasaki; H Koike; M Nishiyama; Y Koyama; M Tanokura

doi:10.1006/jsbi.1997.3906

Crystallization and preliminary crystallographic analysis of the sarcosine oxidase from Bacillus sp. NS-129

J Struct Biol. 1997 Oct;120(1):109-11. doi: 10.1006/jsbi.1997.3906.

Authors

T Ichikawa¹, H Sasaki, H Koike, M Nishiyama, Y Koyama, M Tanokura

Affiliation

¹ Biotechnology Research Center, University of Tokyo, Japan.

PMID: 9356299
DOI: 10.1006/jsbi.1997.3906

Abstract

The sarcosine oxidase from Bacillus sp. NS-129 has been crystallized by vapor diffusion using ammonium sulfate as precipitant. The tetragonal crystals have P4(1)2(1)2 (or P4(3)2(1)2) symmetry with a = b = 177.0 and c = 72.6 A. The crystals probably contain two sarcosine oxidase molecules per asymmetric unit and diffract to at least 2.7 A resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ammonium Sulfate
Bacillus / enzymology*
Crystallization
Crystallography, X-Ray / methods
Oxidoreductases, N-Demethylating / chemistry*
Oxidoreductases, N-Demethylating / isolation & purification
Sarcosine Oxidase

Substances

Oxidoreductases, N-Demethylating
Sarcosine Oxidase
Ammonium Sulfate