Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein

J Klausen; V Pérez; S B Giese; J F García Marín; P Ahrens

doi:10.1016/s0378-1135(97)00133-8

Immunological detection of sheep experimentally infected with strains of Mycobacterium avium subspecies containing insertion sequence IS901/IS902 and a 40 kDa protein

Vet Microbiol. 1997 Sep;57(2-3):181-7. doi: 10.1016/s0378-1135(97)00133-8.

Authors

J Klausen¹, V Pérez, S B Giese, J F García Marín, P Ahrens

Affiliation

¹ Danish Veterinary Laboratory, Copenhagen, Denmark. jk@svs.dk

PMID: 9355253
DOI: 10.1016/s0378-1135(97)00133-8

Abstract

A monoclonal antibody raised against a 40 kDa protein present in certain M. avium strains (IS901/IS902 positive) was used for developing a blocking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with different subspecies of M. avium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Bacterial / blood*
Antibodies, Monoclonal
DNA Transposable Elements*
Enzyme-Linked Immunosorbent Assay
Mutagenesis, Insertional
Mycobacterium avium* / genetics
Sheep
Sheep Diseases*
Tuberculosis / diagnosis
Tuberculosis / microbiology
Tuberculosis / veterinary*

Substances

Antibodies, Bacterial
Antibodies, Monoclonal
DNA Transposable Elements