The reproducibility of gait variables for sound horses is essential for the interpretation of their modifications by locomotor disorders. Therefore, the purpose of this study was to evaluate the variability of the limb joint angle patterns in a population of sound horses while they trotted in the conditions of the routine lameness examination (slow trot, just held by hand, on a track, outdoors). The kinematics of 14 French Saddle horses was recorded using a 3-dimensional (3D) kinematic analysis system. Angle-time diagrams were established in the sagittal plane and their intra- and inter-individual variabilities were evaluated for the whole stride. Horses spontaneously repeated tests at constant speed. The intra- and inter-individual variabilities were low. These values were different from one joint to another and they increased distally. The maximum values were reached by the fetlock and coffin joints. Inter-individual compared to intra-individual variability was 0.9 to 1.9 times greater. There was a high correlation between mean angle-time and mean individual diagrams established for the whole population. It was concluded that it is possible to repeat similar tests with horses trotting in the conditions of the lameness examination. In an homogeneous population, each horse adopts a constant angular pattern that is very close to that of other horses. Since mean angle-time diagrams established on a sound population are highly representative of each mean individual angle pattern, they can be considered as reference data for the further analysis of the pathological gait.