Molecular sex determination and aDNA typings were carried out on a group of five prehistoric individuals. Due to the burial situation and the individual ages the group was assumed to be a family consisting of the parents and three children. DNA was extracted from teeth and bone samples of all skeletons. The aDNA typings based on the PCR amplification of four different microsatellite DNA loci, so-called human short tandem repeats (STR). Results of the molecular sex determinations and allele determinations are presented and compared with morphological determinations. Up to now, the individual biological kinship could be verified for three of the individuals. This is the first proof of individual biological kinship on the molecular level for prehistoric individuals.