Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae

M F van Iersel; M H Eppink; W J van Berkel; F M Rombouts; T Abee

doi:10.1128/aem.63.10.4079-4082.1997

Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae

Appl Environ Microbiol. 1997 Oct;63(10):4079-82. doi: 10.1128/aem.63.10.4079-4082.1997.

Authors

M F van Iersel¹, M H Eppink, W J van Berkel, F M Rombouts, T Abee

Affiliation

¹ Department of Food Science and Nutrition, Wageningen Agricultural University, The Netherlands.

Abstract

An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a molecular mass of 37 kDa and a pI of 5.9. A possible role of the enzyme in flavor production during alcoholic fermentation is discussed.

MeSH terms

Alcohol Oxidoreductases / chemistry
Alcohol Oxidoreductases / isolation & purification*
Alcohol Oxidoreductases / metabolism
Alcoholic Beverages
Anaerobiosis
Fermentation
Food Technology
Hydrogen-Ion Concentration
Isoelectric Point
Molecular Weight
NADP / metabolism
Osmolar Concentration
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / growth & development
Substrate Specificity

Substances

NADP
Alcohol Oxidoreductases
alcohol dehydrogenase (NAD(P)+)