Calcium intake and physical activity level (PAL) were assessed by questionnaire in 124 healthy women aged 52-62 y to determine the effect of calcium intake and PAL on bone mass and turnover. Four groups were identified according to their different reported calcium intakes and PALs. Bone mineral density (BMD) at the spine, hip, and left os calcis was measured together with total bone mineral content (TBMC) with dual-energy X-ray absorptiometry. Bone formation and resorption biochemical markers were measured in fasting samples of blood and urine. Women with the highest calcium intakes and PALs had the highest BMD at all sites compared with those with the lowest calcium intakes and PALs (P < 0.001). Calcium intake and PAL were positively correlated with BMD at all sites. Bone turnover markers did not explain the variation in bone mass between groups. In stepwise-multiple-regression analysis only calcium intake, physical activity, age, or weight remained as independent predictors of BMD and TBMC. When subjects were divided by past PALs, calcium intake and PAL were second to age and weight in their influence on spinal and hip BMD, but remained influential on the os calcis and whole body. We conclude that current high calcium intakes and PALs influence BMD at the os calcis and TBMC and protect bone mass in women 5-12 y postmenopausal at all measured sites, including the spine and hip. This finding does not exclude the possibility of past influences of calcium and activity on bone mass.