We investigated the results of urine culture between the mid-urine and the bladder puncture urine in 30 cases of diabetes millitus. The results showed that female elderly NIDDM and the patients with diabetic retionopathy, nephropathy and neuropathy had higher incidence of urinary infections. When the leukocyte count in routine urine examination was more than 10 increases/HP, the incidence of urinary infection was higher. The urine culture with bladder puncture was more reliable than midurine. In addition, the nuring care for prevention of the urinary infection in diabetes was dicussed.