In higher plants, shoots show negative gravitropism and roots show positive gravitropism. To elucidate the molecular mechanisms of root and hypocotyl gravitropism, we segregated the second mutation from the original phyB-1 mutant line which impaired both root and hypocotyl gravitropism and characterized this novel mutation named rhg (for root and hypocotyl gravitropism). The rhg is a single recessive nuclear mutation and it is mapped on the lower part of the chromosome 1. Analyses on the gravitropic responses of the rhg mutant indicate that root and hypocotyl gravitropism are severely impaired but inflorescence stem gravitropism is not affected by the rhg mutation. In the rhg mutant seedlings, amyloplasts (statoliths for gravity-perception) were present in the presumptive statocytes of roots and hypocotyls. Phototropism by roots and hypocotyls was not impaired in the rhg mutant. These results suggest that the RHG gene product probably acts on the gravity-perception and/or the gravity-signal transduction in root and hypocotyl gravitropism. This is the first report about the genetic locus specifically involved in both root and hypocotyl gravitropism but not inflorescence stem gravitropism, supporting our hypothesis that the mechanisms of gravitropism are genetically different between hypocotyls and inflorescence stems.